Journal: Neural Regeneration Research
Article Title: Regenerative peripheral nerve interface prevents neuroma formation after peripheral nerve transection
doi: 10.4103/1673-5374.353498
Figure Lengend Snippet: Effect of the RPNI on the inhibition of myofibroblast activity in the PNS. (A–F) The immunohistochemistry results of α-SMA expression in the PNSs at 4 and 8 post-surgery. Red arrows indicate α-SMA (brown) expression in myofibroblasts, and red arrowheads indicate α-SMA expression in the vascular smooth muscle. The percentage of the α-SMA-positive area in the RPNI group was significantly lower than that in the NSM and control groups at 8 weeks post-surgery. Scale bars: 50 μm. (G, H) Statistical analyses of the percentages of the α-SMA-positive area at 4 (G) and 8 (H) weeks post-surgery in the three groups. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test). NSM: Nerve stump implantation inside the muscle; PNS: proximal nerve stump; RPNI: regenerative peripheral nerve interface; α-SMA: α-smooth muscle actin.
Article Snippet: For the immunohistochemistry assay, an anti-α-SMA antibody (fibrosis marker; mouse, 1:200, Boster, Wuhan, China, Cat# BM0002, RRID: AB_2811044) was used to evaluate myofibroblast activity, and an anti-S1R antibody (rabbit; 1:500, Proteintech, Beijing, China, Cat# 15168-1-AP, RRID: AB_2301712) was used to examine neuroinflammation in the PNS.
Techniques: Inhibition, Activity Assay, Immunohistochemistry, Expressing, Control